Translational_Unit
Part:BBa_K953001:Design
Designed by: Daniel Winter Group: iGEM12_Macquarie_Australia (2012-09-23)
Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 919
Illegal BamHI site found at 724 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 337
Illegal NgoMIV site found at 1258
Illegal AgeI site found at 524
Illegal AgeI site found at 569
Illegal AgeI site found at 1630 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimised for expression in E. coli
Source
Deinococcus radiodurans